Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...

Saccharomyces cerevisiae has long been grown in fed-batch cultivation by the brewing and baking industry, where it was selected principally for its vigorous growth. It has also been used in the ...

Pichia pastoris has emerged as a leading eukaryotic host for recombinant protein production, owing to its ability to perform complex post-translational modifications and grow to high cell densities ...

Proteins are bio-macromolecules with a range of functionalities as a result of complex higher-order structures. As the end product of the “central dogma” in biology, proteins are considered the key ...